Importance of co-cultivation medium pH for successful Agrobacterium-mediated transformation of Lilium x formolongi.
Identifieur interne : 000677 ( Main/Exploration ); précédent : 000676; suivant : 000678Importance of co-cultivation medium pH for successful Agrobacterium-mediated transformation of Lilium x formolongi.
Auteurs : M. Ogaki [Japon] ; Y. Furuichi ; K. Kuroda ; D P Chin ; Y. Ogawa ; M. MiiSource :
- Plant cell reports [ 0721-7714 ] ; 2008.
Descripteurs français
- KwdFr :
- Concentration en ions d'hydrogène (MeSH), Lilium (croissance et développement), Lilium (génétique), Lilium (microbiologie), Milieux de culture (MeSH), Méristème (croissance et développement), Méristème (microbiologie), Méristème (physiologie), Rhizobium (métabolisme), Vecteurs génétiques (MeSH), Végétaux génétiquement modifiés (croissance et développement), Végétaux génétiquement modifiés (microbiologie), Végétaux génétiquement modifiés (physiologie).
- MESH :
- croissance et développement : Lilium, Méristème, Végétaux génétiquement modifiés.
- génétique : Lilium.
- microbiologie : Lilium, Méristème, Végétaux génétiquement modifiés.
- métabolisme : Rhizobium.
- physiologie : Méristème, Végétaux génétiquement modifiés.
- Concentration en ions d'hydrogène, Milieux de culture, Vecteurs génétiques.
English descriptors
- KwdEn :
- Culture Media (MeSH), Genetic Vectors (MeSH), Hydrogen-Ion Concentration (MeSH), Lilium (genetics), Lilium (growth & development), Lilium (microbiology), Meristem (growth & development), Meristem (microbiology), Meristem (physiology), Plants, Genetically Modified (growth & development), Plants, Genetically Modified (microbiology), Plants, Genetically Modified (physiology), Rhizobium (metabolism).
- MESH :
- chemical : Culture Media.
- genetics : Lilium.
- growth & development : Lilium, Meristem, Plants, Genetically Modified.
- metabolism : Rhizobium.
- microbiology : Lilium, Meristem, Plants, Genetically Modified.
- physiology : Meristem, Plants, Genetically Modified.
- Genetic Vectors, Hydrogen-Ion Concentration.
Abstract
An efficient system for Agrobacterium-mediated transformation of Lilium x formolongi was established by preventing the drastic drop of pH in the co-cultivation medium with MES. Meristematic nodular calli were inoculated with an overnight culture of A. tumefaciens strain EHA101 containing the plasmid pIG121-Hm which harbored intron-containing beta-glucuronidase (GUS), hygromycin phosphotransferase (HPT), and neomycin phosphotransfease II (NPTII) genes. After three days of co-cultivation on 2 g/l gellan gum-solidified MS medium containing 100 microM acetosyringone, 30 g/l sucrose, 1 mg/l picloram and different concentrations of MES, they were cultured on the same medium containing 12.5 mg/l meropenem to eliminate Agrobacterium for 2 weeks and then transferred onto medium containing the same concentration of meropenem and 25 mg/l hygromycin for selecting putative transgenic calli. Transient GUS expression was only observed by adding MES to co-cultivation medium. Hygromycin-resistant transgenic calli were obtained only when MES was added to the co-cultivation medium especially at 10 mM. The hygromycin-resistant calli were successfully regenerated into plantlets after transferring onto picloram-free medium. Transformation of plants was confirmed by histochemical GUS assay, PCR analysis and Southern blot analysis.
DOI: 10.1007/s00299-007-0481-x
PubMed: 18060407
Affiliations:
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Kuroda</name></author><author><name sortKey="Chin, D P" sort="Chin, D P" uniqKey="Chin D" first="D P" last="Chin">D P Chin</name></author><author><name sortKey="Ogawa, Y" sort="Ogawa, Y" uniqKey="Ogawa Y" first="Y" last="Ogawa">Y. Ogawa</name></author><author><name sortKey="Mii, M" sort="Mii, M" uniqKey="Mii M" first="M" last="Mii">M. 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Ogaki</name><affiliation wicri:level="1"><nlm:affiliation>Graduate School of Horticulture, Chiba University,Matsudo, Chiba, 271-8510, Japan. ogaki-mmk@msb.biglobe.ne.jp</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Graduate School of Horticulture, Chiba University,Matsudo, Chiba, 271-8510</wicri:regionArea><wicri:noRegion>271-8510</wicri:noRegion></affiliation></author><author><name sortKey="Furuichi, Y" sort="Furuichi, Y" uniqKey="Furuichi Y" first="Y" last="Furuichi">Y. Furuichi</name></author><author><name sortKey="Kuroda, K" sort="Kuroda, K" uniqKey="Kuroda K" first="K" last="Kuroda">K. Kuroda</name></author><author><name sortKey="Chin, D P" sort="Chin, D P" uniqKey="Chin D" first="D P" last="Chin">D P Chin</name></author><author><name sortKey="Ogawa, Y" sort="Ogawa, Y" uniqKey="Ogawa Y" first="Y" last="Ogawa">Y. Ogawa</name></author><author><name sortKey="Mii, M" sort="Mii, M" uniqKey="Mii M" first="M" last="Mii">M. Mii</name></author></analytic><series><title level="j">Plant cell reports</title><idno type="ISSN">0721-7714</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Culture Media (MeSH)</term><term>Genetic Vectors (MeSH)</term><term>Hydrogen-Ion Concentration (MeSH)</term><term>Lilium (genetics)</term><term>Lilium (growth & development)</term><term>Lilium (microbiology)</term><term>Meristem (growth & development)</term><term>Meristem (microbiology)</term><term>Meristem (physiology)</term><term>Plants, Genetically Modified (growth & development)</term><term>Plants, Genetically Modified (microbiology)</term><term>Plants, Genetically Modified (physiology)</term><term>Rhizobium (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Concentration en ions d'hydrogène (MeSH)</term><term>Lilium (croissance et développement)</term><term>Lilium (génétique)</term><term>Lilium (microbiologie)</term><term>Milieux de culture (MeSH)</term><term>Méristème (croissance et développement)</term><term>Méristème (microbiologie)</term><term>Méristème (physiologie)</term><term>Rhizobium (métabolisme)</term><term>Vecteurs génétiques (MeSH)</term><term>Végétaux génétiquement modifiés (croissance et développement)</term><term>Végétaux génétiquement modifiés (microbiologie)</term><term>Végétaux génétiquement modifiés (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Culture Media</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Lilium</term><term>Méristème</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Lilium</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Lilium</term><term>Meristem</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Lilium</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Rhizobium</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Lilium</term><term>Méristème</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Lilium</term><term>Meristem</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Rhizobium</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Méristème</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Meristem</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Genetic Vectors</term><term>Hydrogen-Ion Concentration</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Concentration en ions d'hydrogène</term><term>Milieux de culture</term><term>Vecteurs génétiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">An efficient system for Agrobacterium-mediated transformation of Lilium x formolongi was established by preventing the drastic drop of pH in the co-cultivation medium with MES. Meristematic nodular calli were inoculated with an overnight culture of A. tumefaciens strain EHA101 containing the plasmid pIG121-Hm which harbored intron-containing beta-glucuronidase (GUS), hygromycin phosphotransferase (HPT), and neomycin phosphotransfease II (NPTII) genes. After three days of co-cultivation on 2 g/l gellan gum-solidified MS medium containing 100 microM acetosyringone, 30 g/l sucrose, 1 mg/l picloram and different concentrations of MES, they were cultured on the same medium containing 12.5 mg/l meropenem to eliminate Agrobacterium for 2 weeks and then transferred onto medium containing the same concentration of meropenem and 25 mg/l hygromycin for selecting putative transgenic calli. Transient GUS expression was only observed by adding MES to co-cultivation medium. Hygromycin-resistant transgenic calli were obtained only when MES was added to the co-cultivation medium especially at 10 mM. The hygromycin-resistant calli were successfully regenerated into plantlets after transferring onto picloram-free medium. Transformation of plants was confirmed by histochemical GUS assay, PCR analysis and Southern blot analysis.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18060407</PMID><DateCompleted><Year>2008</Year><Month>08</Month><Day>18</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0721-7714</ISSN><JournalIssue CitedMedium="Print"><Volume>27</Volume><Issue>4</Issue><PubDate><Year>2008</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Plant cell reports</Title><ISOAbbreviation>Plant Cell Rep</ISOAbbreviation></Journal><ArticleTitle>Importance of co-cultivation medium pH for successful Agrobacterium-mediated transformation of Lilium x formolongi.</ArticleTitle><Pagination><MedlinePgn>699-705</MedlinePgn></Pagination><Abstract><AbstractText>An efficient system for Agrobacterium-mediated transformation of Lilium x formolongi was established by preventing the drastic drop of pH in the co-cultivation medium with MES. Meristematic nodular calli were inoculated with an overnight culture of A. tumefaciens strain EHA101 containing the plasmid pIG121-Hm which harbored intron-containing beta-glucuronidase (GUS), hygromycin phosphotransferase (HPT), and neomycin phosphotransfease II (NPTII) genes. After three days of co-cultivation on 2 g/l gellan gum-solidified MS medium containing 100 microM acetosyringone, 30 g/l sucrose, 1 mg/l picloram and different concentrations of MES, they were cultured on the same medium containing 12.5 mg/l meropenem to eliminate Agrobacterium for 2 weeks and then transferred onto medium containing the same concentration of meropenem and 25 mg/l hygromycin for selecting putative transgenic calli. Transient GUS expression was only observed by adding MES to co-cultivation medium. Hygromycin-resistant transgenic calli were obtained only when MES was added to the co-cultivation medium especially at 10 mM. The hygromycin-resistant calli were successfully regenerated into plantlets after transferring onto picloram-free medium. Transformation of plants was confirmed by histochemical GUS assay, PCR analysis and Southern blot analysis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ogaki</LastName><ForeName>M</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Graduate School of Horticulture, Chiba University,Matsudo, Chiba, 271-8510, Japan. ogaki-mmk@msb.biglobe.ne.jp</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Furuichi</LastName><ForeName>Y</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Kuroda</LastName><ForeName>K</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Chin</LastName><ForeName>D P</ForeName><Initials>DP</Initials></Author><Author ValidYN="Y"><LastName>Ogawa</LastName><ForeName>Y</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Mii</LastName><ForeName>M</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2007</Year><Month>12</Month><Day>04</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Plant Cell Rep</MedlineTA><NlmUniqueID>9880970</NlmUniqueID><ISSNLinking>0721-7714</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003470">Culture Media</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D003470" MajorTopicYN="N">Culture Media</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005822" MajorTopicYN="N">Genetic Vectors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006863" MajorTopicYN="N">Hydrogen-Ion Concentration</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D027762" MajorTopicYN="N">Lilium</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018519" MajorTopicYN="N">Meristem</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012231" MajorTopicYN="N">Rhizobium</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2007</Year><Month>07</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2007</Year><Month>11</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2007</Year><Month>11</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2007</Year><Month>12</Month><Day>7</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2008</Year><Month>8</Month><Day>19</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2007</Year><Month>12</Month><Day>7</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">18060407</ArticleId><ArticleId IdType="doi">10.1007/s00299-007-0481-x</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Plant Mol Biol. 1997 Sep;35(1-2):205-18</Citation><ArticleIdList><ArticleId IdType="pubmed">9291974</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1980 Oct 10;8(19):4321-5</Citation><ArticleIdList><ArticleId IdType="pubmed">7433111</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2002 Sep 17;99(19):12369-74</Citation><ArticleIdList><ArticleId IdType="pubmed">12218184</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 1991 Jun;16(6):1051-9</Citation><ArticleIdList><ArticleId 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IdType="pubmed">9630983</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1993 Jun;102(2):357-361</Citation><ArticleIdList><ArticleId IdType="pubmed">12231826</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Rep. 1996 Jun;15(10):727-30</Citation><ArticleIdList><ArticleId IdType="pubmed">24178159</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Japon</li></country></list><tree><noCountry><name sortKey="Chin, D P" sort="Chin, D P" uniqKey="Chin D" first="D P" last="Chin">D P Chin</name><name sortKey="Furuichi, Y" sort="Furuichi, Y" uniqKey="Furuichi Y" first="Y" last="Furuichi">Y. Furuichi</name><name sortKey="Kuroda, K" sort="Kuroda, K" uniqKey="Kuroda K" first="K" last="Kuroda">K. Kuroda</name><name sortKey="Mii, M" sort="Mii, M" uniqKey="Mii M" first="M" last="Mii">M. Mii</name><name sortKey="Ogawa, Y" sort="Ogawa, Y" uniqKey="Ogawa Y" first="Y" last="Ogawa">Y. Ogawa</name></noCountry><country name="Japon"><noRegion><name sortKey="Ogaki, M" sort="Ogaki, M" uniqKey="Ogaki M" first="M" last="Ogaki">M. Ogaki</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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